GC Analysis of Terpenes in Hops (US Goldings) on Equity®-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber

GC Analysis of Terpenes in Hops (US Goldings) on Equity®-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber

Conditions

sample preparation 30 min equilibration at 40 C prior to extraction
sample preparation fiber postbake after extraction, 3 min at 270 C
sample/matrix ground US Golding hop pellets
desorption process 3 min, 270 C
extraction 20 min, headspace, 40 C
SPME fiber Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U)
column Equity-1, 60 m x 0.25 mm I.D., 0.25 µm (28047-U)
oven 60 C (2 min), 5 C/min to 275 C (5 min)
carrier gas helium, 1 ml/min constant flow
liner 0.75 mm ID, SPME
inj. temp. 270 C
detector MSD
MSD interface 300 C

Description

Analysis Note Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in US Goldings variety of hops. Chromatographic separation was performed on an Equity®-1 capillary GC column, and identification was done using retention indices and spectral library match. Analysis showed a characteristic terpene profile, with high levels of ß-myrcene, caryophyllene, and humulene present.
Categories Analytical Chromatography, GC Applications, Terpenes, Hydrocarbons
Featured Industry Food and Beverages
Legal Information Equity is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes add 0.5 g ground hops to a 10 mL headspace vial
suitability application for SPME, application for GC

Materials

     
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