GC Analysis of Volatiles in Limoncello on SLB®-5ms after SPME using a 100 μm PDMS Fiber

GC Analysis of Volatiles in Limoncello on SLB®-5ms after SPME using a 100 μm PDMS Fiber

Conditions

sample/matrix Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 µl of a 100 ppm solution of nonane (internal standard)
desorption process 250 C for 5 min
extraction headspace, with a pre-equilibration period of 10 min. at 40 C, and then a fiber exposure of 30 min. at 40 C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm
SPME fiber 100 µm polydimethylsiloxane (57300-U)
column SLB-5ms, 30 m x 0.25 mm I.D., 0.25 µm (28471-U)
oven 40 C, 3 C/min to 250 C (2 min)
carrier gas helium, 35.0 cm/sec
sample Limoncello liquor
injection splitless
liner 0.75 mm I.D. SPME (2633901)
inj. temp. 250 C
detector FID, 260 C (sampling rate 80 ms)

Description

Analysis Note In this application, SPME was used for the analysis of volatiles in limoncello using a headspace method. SPME is ideal for the analysis of aroma volatiles in flavored beverages such as liquors. A 100 μm PDMS fiber was inserted into the headspace of the limoncello sample for 30 minutes at 40 °C followed by GC analysis using FID detection. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.
Categories Analytical Chromatography, Food & Beverage Analysis, SPME Applications, GC Applications, Foods, Flavors & Fragrances, Beverage Testing, Volatiles, Beer, Wine, and Spirits, Spirits
Featured Industry Food and Beverages
Legal Information SLB is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Analyte

  • (E)-Caryophyllene
  • (E)-α-Bisabolene
  • (E)-β-Ocimene
  • (E)-γ-Bisabolene
  • (Z)-β-Ocimene
  • (Z)-β-Santelene
  • Bicycloelemene
  • Bicyclogermacrene
  • Camphene
  • Campherenol
  • cis-Limonene oxide
  • cis-Sabinene hydrate
  • Citronellal
  • Citronellyl acetate
  • Decanal
  • Geranial
  • Geraniol
  • Geranyl acetate
  • Geranyl propionate
  • Germacrene D
  • Isobornyl acetate
  • Limonene
  • Linalool
  • Methyl geranoate
  • Myrcene
  • Neral
  • Neryl acetate
  • Nonanal
  • Nonyl acetate
  • Octanol
  • Octyl acetate
  • p-Cymene
  • Perilla alcohol
  • Perilla aldehyde
  • Sabinene
  • Terpinen-4-ol
  • Terpinolene
  • trans-α-Bergamotene
  • Tricyclene
  • Undecanal
  • Valencene
  • α-Bisabolol
  • α-cis-Bergamotene
  • α-Huµlene
  • α-Phellandrene
  • α-Pinene
  • α-Terpinene
  • α-Terpineol
  • α-Thujene
  • β-bisabolene
  • β-Pinene
  • γ-Terpinene
  • δ-3-Carene
  • δ-Cadinene

Materials

     
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