GC Analysis of Volatiles in Multifloral Honey on SUPELCOWAX® 10 after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber

GC Analysis of Volatiles in Multifloral Honey on SUPELCOWAX® 10 after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber

Conditions

sample/matrix 5 g multifloral honey + 5 mL bidistilled water in a 20 mL vial, allowed to equilibrate at 50 C for 15 min with magnetic stirring
desorption process 250 C for 2 min
extraction headspace, 50 C for 40 min with magnetic stirring
SPME fiber 2 cm 50/30 µm divinylbenzene/carboxen on polydimethylsiloxane (57348-U)
column SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 µm (24079)
oven 35 C (8 min), 6 C/min to 60 C, 4 C/min to 160 C, 20 C/min to 200 C (1 min)
carrier gas helium, 1 ml/min
sample (Honey)
liner 0.75 mm I.D., SPME
inj. temp. 250 C
MSD interface 230 C
scan range 35-300 m/z

Description

Analysis Note In this application, headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.
Categories Analytical Chromatography, SPME Applications, GC Applications, Agriculture, Foods, Flavors & Fragrances, Volatiles
Featured Industry Agriculture
Food and Beverages
Legal Information SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
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Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Analyte

  • 3-Phenylfuran
  • Benzaldehyde
  • Decanal
  • Furfural
  • Hotrienol
  • Nonanal
  • Nonanoic acid
  • Nonanol
  • Phenylacetaldehyde
  • Phenylethyl alcohol
  • α-Linalool

Materials

     
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