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GC Analysis of Water in Ibuprofen (Spiked) on Watercol™ 1910

GC Analysis of Water in Ibuprofen (Spiked) on Watercol™ 1910

Conditions

column Watercol 1910, 30 m x 0.25 mm I.D., 0.20 µm (29711-U)
oven 35 C (4 min), 8 C/min to 130 C (1 min)
carrier gas helium, 11.40 psi constant pressure (equal to 25 cm/sec), measured with oven at 35 C
sample 0.198 g ibuprofen weighed into a 1 mL volumetric flask, 0.5 mL acetone added, vortexed slowly for 30 seconds, acetone added to the 1 mL line, vortexed, transferred to an autosampler vial
injection 1 µL, 100:1 split
liner 4 mm I.D., split/splitless type, wool packed single taper FocusLiner(TM) design (2879925-U)
inj. temp. 250 C
detector TCD, 200

Description

Analysis Note Residual moisture in an active pharmaceutical ingredient (API) is undesirable. This is because it dilutes the amount of the API. It may also interfere with the performance of the API.

The "Watercol" series of ionic liquid capillary GC columns are characterized by their ability to produce a sharp peak shape for water, sharp enough so that water can be integrated and quantified. This application shows the suitability of Watercol 1910 for the analysis of water in ibuprofen. The water peak was confirmed by injecting a series of standards (water in acetone).
Featured Industry Pharmaceutical (small molecule)
Legal Information Watercol is a trademark of Sigma-Aldrich Co. LLC
suitability application for GC

Materials

     
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