GCxGC Analysis of Brazilian Cachaça on SLB®-IL59 x 5%Phenyl Column after SPME using 50/30 μm DVB/Carboxen®/PDMS and Overcoated 50/30 μm DVB/Carboxen/PDMS Fibers, Polar to Non-Polar Strategy, Apex Plots

GCxGC Analysis of Brazilian Cachaça on SLB®-IL59 x 5%Phenyl Column after SPME using 50/30 μm DVB/Carboxen®/PDMS and Overcoated 50/30 μm DVB/Carboxen/PDMS Fibers, Polar to Non-Polar Strategy, Apex Plots

Conditions

sample/matrix 9 mL cachaça in a 20 mL vial, pre-heated at 30 °C for 15 min with agitation, no salt added
desorption process 250 °C for 25 min
extraction (Headspace) 30 °C for 60 min with agitation; (Direct Immersion) 30 °C for 60 min with agitation, rapid rinse of SPME fiber in deionized water before desorption
SPME fiber (Direct Immersion) Overcoated 50/30 µm DVB/Carboxen/PDMS on 1 cm StableFlex (custom)
SPME fiber (Headspace) 50/30 µm DVB/Carboxen®/PDMS on 1 cm StableFlex™ (57298-U)
column (1D) SLB-IL59, 30 m x 0.25 mm I.D., 0.20 µm (28891-U)
column (2D) 5% phenyl polysiloxane, 2 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, SLB-5ms, 28469-U, 2 m length cut from 15 m column)
oven (2D) 85 °C (3 min), 3 °C/min to 330 °C (5 min)
oven (1D) 50 °C (3 min), 3 °C/min to 295 °C (5 min)
oven (modulator) temp. offset 35 °C, modulation period 10 sec
carrier gas helium, 1.0 mL/min
injection 30:1 split
liner 0.75 mm I.D., direct (SPME) type, straight design
inj. temp. 250 °C
detector TOFMS, 250 °C, m/z = 45-400
MSD interface 200 °C

Description

Categories Analytical Chromatography, GC Applications, Volatiles, Semivolatiles
Featured Industry Food and Beverages
Legal Information Carboxen is a registered trademark of Sigma-Aldrich Co. LLC, SLB is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPME, application for GC

Materials

     
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