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GCxGC Analysis of FAMEs in Human Plasma on Equity®-1 x SUPELCOWAX® 10, Non-Polar to Polar Strategy

GCxGC Analysis of FAMEs in Human Plasma on Equity®-1 x SUPELCOWAX® 10, Non-Polar to Polar Strategy

Conditions

column (1D) Equity 1, 30 m x 0.25 mm I.D., 0.25 µm (28406-U)
column (2D) SUPELCOWAX 10, 0.95 m x 0.10 mm I.D., 0.10 µm (24343)
oven 180 C, 3 C/min to 280 C (10 min)
oven (modulator) LMCS Everest longitudinally modulated cryogenic system, modulation period 6 sec
carrier gas hydrogen
sample human plasma
inj. temp. 280 C
detector FID, 300 C, 125Hz; MS, 20000 amu/sec

Description

Analysis Note Sample prep: 100 mL of plasma was saponified with 1 mL of methanolic sodium methoxide (0.5% w/v) at a temperature of 100 °C for 15 min in a closed Pyrex tube. The subsequent methylic esterification was achieved with 1 mL boron trifluoride-methanol complex at a temperature of 100 °C for 15 min. The fatty acid methyl esters (FAMEs) were extracted by adding 1 mL of n-hexane to the mixture. One mL of a saturated NaCl solution was added to the entire mixture, which was then agitated manually for 2 min, followed by centrifugation at 3000 rpm for 10 min. After, anhydrous Na2SO4 was added to the Pyrex tube for drying and 0.9 mL of the n-hexane FAME layer was transferred to a GC injector vial. A 0.1 mL volume of 1000 ppm internal standard (C13:0) was also added to the GC vial.
Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Clinical
General description The 0.95 m SUPELCOWAX 10 column was obtained by cutting lengths from a standard length column (e.g. 5, 10, 15 m).
Legal Information SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC, Equity is a registered trademark of Sigma-Aldrich Co. LLC
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Materials

     
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