SFC Analysis of Warfarin™ Enantiomers on Astec® Cellulose DMP, Methanol Additive

SFC Analysis of Warfarin™ Enantiomers on Astec® Cellulose DMP, Methanol Additive

Conditions

column Astec Cellulose DMP, 15 cm x 4.6 mm I.D., 5 µm particles (51098AST)
column temp. 35 °C
mobile phase [A] CO2; [B] methanol; (80:20, A:B)
flow rate 3 mL/min
pressure 1450 psi (100 bar)
sample warfarin enantiomers, 2 mg/mL
injection 5 µL
detector UV, 220 nm

Description

Analysis Note SFC data provided by Dr. Christina Kraml, Lotus Separations, LLC, Princeton, NJ.
Instrument: Berger Analytical SFCsystem equipped with a dual pump (FCM-1200), autosampler (ALS 3100), column oven (TCM-200) and diode-array detector (DAD-4100); from Mettler Toledo.
Categories Analytical Chromatography, SFC Applications, Chiral Applications, Clinical, Forensic & Toxicology, Therapeutic Drug Monitoring
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Warfarin is a trademark of Wisconsin Alumni Research Foundation, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SFC

Materials

     
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