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EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA) on SUPELCOSIL™ SAX1

EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA) on SUPELCOSIL™ SAX1

Conditions

column SUPELCOSIL SAX1, 25 cm x 4.6 mm I.D., 5 µm particles (59138)
column temp. 38 C
mobile phase [A] 5 mM monobasic potassium phosphate (pH 1.9 with phosphoric acid); [B] methanol; (A:B, 96:4)
flow rate 0.5 ml/min
sample 100 ng/mL in water
injection 100 µl
detector fluorescence, excitation 338 nm, emission 455 nm
detector post column conditions for the oxidation solution (see EPA Method 547): flow rate  0.2 ml/min, temperature 38 C, reactor volume 500 µl; (post column conditions for the derivatization solution (see EPA Method 547): flow rate 0.5 ml/min, ambient temperature, mixing device 500 µl mixing loop (0.5 mm))

Description

Analysis Note EPA Method 547 outlines the analysis of glyphosate in drinking water by direct aqueous injection HPLC, post column derivatization, and fluorescence detection. Anion exchange columns can be used for this method and have been shown to produce excellent results. A system is described here using a silica-based anion-exchange HPLC column (SUPELCOSIL SAX1) instead of a polymer-based column. Advantages of the silica-based column include compatibility with organic solvents and higher pressure stability. These, in turn, lead to increased column lifetime.
Categories Analytical Chromatography, Food & Beverage Analysis, Beverage Testing, Drinking Water and Bottled Water
Featured Industry Environmental
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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