HPLC (1D-Ag+-LC-ELSD) Analysis of Triacylglycerols in Gilthead Bream (Sparus aurata) on Nucleosil® SA following Extraction by Folch, Bligh & Dyer, and Maceration Methods

HPLC (1D-Ag+-LC-ELSD) Analysis of Triacylglycerols in Gilthead Bream (Sparus aurata) on Nucleosil® SA following Extraction by Folch, Bligh & Dyer, and Maceration Methods

Conditions

column Nucleosil SA (sulfonic acid), 25 cm x 4.6 mm I.D., 5 µm (50174-U)
mobile phase [A] 0.5% butyronitrile (BCN) in hexane; [B] 5% butyronitrile (BCN) in hexane
gradient 0 to 100% B in 100 min
flow rate 0.8 mL/min
sample fish tissue extracted by Folch, Bligh & Dyer, and maceration methods
injection 10 µL
detector ELSD

Description

Analysis Note Data provided by Marco Beccaria (1) and Luigi Mondello (1,2) of (1) Chromaleont s.r.l., c/o “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy or (2) “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy.

Triacylglycerols extracted from fish tissue were analyzed by an off-line combination of silver-ion liquid chromatography using a Nucleosil SA column and non-aqueous reversed-phase liquid chromatography (NARP) using an Ascentis Express C18 column. Shown here is the first dimension separation on the Nucleosil SA column. PN is the carbon number (CN) minus twice the double bond number (DBN).
Featured Industry Food and Beverages
Legal Information Nucleosil is a registered trademark of Macherey-Nagel GmbH & Co. KG
suitability application for HPLC

Materials

     
Related Links