HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide

HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 15 cm x 3 mm I.D., 2.7 µm particles (53919-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile
gradient 5 to 53% B in 70 min
flow rate 0.4 ml/min
sample 20 mg/L (except terbuthylazin desethyl and terbuthylazine 60 mg/L) in 60:40, water: acetonitrile
injection 5 µl
detector UV, 240 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of 16 triazine and phenyl urea compounds. Lower column temperature can improve resolution of the critical pair.
Categories Analytical Chromatography, Pesticides & Herbicides
Featured Industry Agriculture
Chemicals and Industrial Polymers
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links