HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 40 °C

Conditions

column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U)
column temp. 40 °C
mobile phase [A] 7 mM ammonium formate; [B] methanol (25:75, A:B)
flow rate 0.4 mL/min
pressure 3553 psi (245 bar)
sample 300 mg/L in 25:75, water:methanol
injection 1 µL
detector UV, 265 nmESI(+), m/z 100 - 1000

Description

Categories Analytical Chromatography, Clinical, vitamins, Vitamins and Natural products
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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