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HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX

HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix urine spiked with 3- and 4-methylpyrazole at 1 mg/mL
SPE tube/cartridge Discovery DSC-SCX, 500 mg/3 mL (52686-U)
condition 2 mL methanol; 2 mL DI water
sample addition 1 mL
washing 2 mL DI water
elution 2 mL 5% methanol in 250 mM potassium phosphate buffer, pH 7.4
column Discovery C18, 15 cm x 4.6 mm, 5 µm preceded by a 2 cm guard column and 0.5 µm frit filter (504955)
column temp. 30 C
mobile phase MeOH:5 mM phosphate buffer, pH 6 (20:80)
flow rate 1 ml/min
injection 25 µL, dilute urine extract
detector UV, 220 nm

Description

Categories Analytical Chromatography, SPE Applications, Forensics and Toxicology, antidotes, Pharmaceutical & Clinical applications
Featured Industry Forensics and Toxicology
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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