HPLC Analysis of a Monoclonal Antibody Tryptic Digest on BIOshell™ A160 Peptide C18, 2.7 μm

HPLC Analysis of a Monoclonal Antibody Tryptic Digest on BIOshell™ A160 Peptide C18, 2.7 μm

Conditions

sample preparation MSQC4 monoclonal antibody, 0.5 g/L (final), digested with trypsin at high temperature per Perfinity Flash Digest protocol
column BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (66905-U)
mobile phase [A] 98:2, (0.1% TFA in water):(0.09% TFA in acetonitrile); [B] 50:50, (0.1% TFA in water):(0.09% TFA in acetonitrile)
gradient 0 to 63%B in 60 min
flow rate 0.3 mL/min
pressure 4100 psiinitial (total system) (283 bar)
injection 20 µL
detector UV, 215 nm

Description

Analysis Note Intact monoclonal antibody MSQC4 was digested with trypsin (iwthout denaturation or alkylation), and chromatographed on the BIOshell peptide column.
Categories Analytical Chromatography, Peptides
Featured Industry Clinical
Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links