HPLC Analysis of Acidic Herbicides in Water on a Polymeric C18 Column after SPE using Supelclean™ ENVI™-Carb

HPLC Analysis of Acidic Herbicides in Water on a Polymeric C18 Column after SPE using Supelclean™ ENVI™-Carb

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix Fresh 1 L water samples, dechlorinated with sodium thiosulfate when necessary, at ambient temperature and pH.
SPE tube/cartridge Supelclean ENVI-Carb, 250 mg/6 mL (57092)
condition 10 mL DI water at 20 mL/min
sample addition 0.9 L water sample at 20 mL/min
washing 10 mL DI water at 20 mL/min
drying 10 min using clean nitrogen
elution 10 mL 0.1% phosphoric acid in methylene chloride:acetonitrile (80:20) at 5 mL/min
column polymeric-coated silica-based PAH specialty column, 20 cm x 3 mm I.D., 5 µm (Supelco equivalent, SUPELCOSIL LC-PAH, available upon request)
column temp. 50 °C
mobile phase gradient, [A]: 0.05% phosphoric acid in DI water; [B]: acetonitrile
flow rate 0.5 mL/min
injection 10 µLof extract (4-5 ppb each analyte in water)
detector photodiode array- peak width: 0.053 min, sampling interval: 0.320 sec, monitor 210 nm & 225 nm

Description

Application using Zymark AutoTrace Extraction WorkStation 1.20
Categories Analytical Chromatography, Environmental Analysis, SPE Applications, Herbicides, Organics, Chromatograms, HPLC Chromatograms
Featured Industry Environmental
Legal Information Supelclean is a trademark of Sigma-Aldrich Co. LLC, ENVI is a trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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