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HPLC Analysis of Adenosine Nucleosides on Supelcosil™ LC-18-T

HPLC Analysis of Adenosine Nucleosides on Supelcosil™ LC-18-T

Conditions

column SUPELCOSIL LC-18-T, 15 cm x 4.6 mm I.D, 3 µm particles (58970-U)
column temp. 30 C
mobile phase [A] 0.1 M potassium phosphate, pH 7; [B] acetonitrile
gradient 0 to 2.5% B in 4 min, to 7.5% B in 2 min, to 0% B in 0.1 min, held at 0% B for 3.9 min
flow rate 1 ml/min
sample each compound 5 µg/mL in water
injection 20 µl
detector UV, 254 nm

Description

Analysis Note A need to analyze nucleosides separated from plant and animal products for pediatric nutritional and other research interests exists. SUPELCOSIL LC-18-T columns have proven effective in the separation of adenosine nucleosides.
Categories Analytical Chromatography, Nucleosides
Featured Industry Life Science and Biopharma
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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