HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea and Fluorescence Detection

HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea and Fluorescence Detection

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 25 g corn meal in 100 mL acetonitrile:water (84:16)
SPE tube/cartridge Supel(TM) Tox AflaZea, 6 mL (55314-U)
sample addition 2 mL
elution collect sample in 5 mL silanized glass vial
eluate post-treatment transfer 200 µl sample, add 300 µl TFA derivatization solution (70:20:10 water:TFA:acetic acid), heat in microreactor 20 min at 65 C, cool, add 580 µl deionized water, vortex
column Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (53825-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (80:20, A:B)
flow rate 0.350 ml/min
pressure 325 psi (22.4 bar)
injection 20 µl
detector fluorescence, ex 360 nm, em 440 nm

Description

Analysis Note Aflatoxins are closely related compounds produced as secondary metablolites of fungi. They are found in grains and nuts like corn, wheat or peanuts. Shown here is a rapid, sensitive method to extract these compounds from corn using SPE cartridges specifically designed for aflatoxins, Supel Tox AflaZea (aflatoxin zearalenone), which removes interferences from grains, feeds, TMR samples, peanuts, peanut products and aqueous solutions. Analysis was via HPLC on Ascentis Express Fused-Core C18 colums.
Categories Analytical Chromatography, SPE Applications, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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