HPLC Analysis of Aflatoxins on Ascentis® Express C18

HPLC Analysis of Aflatoxins on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 35 C
mobile phase [A] 5 mM ammonium formate, pH 2.5 (titrated with formic acid); [B] methanol
gradient 5 to 100% B in 10 min
flow rate 0.4 ml/min
pressure 4714 psi (325 bar)
sample 0.15 - 30 mg/L in 90:10, water:acetonitrile
injection 5 µl
detector APCI (+)

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of various toxins.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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