HPLC Analysis of Aflatoxins on Ascentis® Express C18 with Fluorescence Detection

HPLC Analysis of Aflatoxins on Ascentis® Express C18 with Fluorescence Detection

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (75:25, A:B)
flow rate 2.0 ml/min
pressure 6381 psi (440 bar)
sample 200 µl aflatoxin standard in acetonitrile; 0.5 µg/mL B2 and G2; 2 µg/mL B1 and G1; 800 µl delivatization solution: 70% water, 20% TFA and 10% acetic acidheat for 9 min at 65 C
injection 50 µl
detector fluorescence, ex 360 nm, em 440 nm

Description

Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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