HPLC Analysis of Aflatoxins on Ascentis® Express C18

HPLC Analysis of Aflatoxins on Ascentis® Express C18


column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 35 C
mobile phase [A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 µL/L nitric acid
flow rate 0.400 ml/min
pressure 4568 psi (315 bar)
sample 200 µl spiked 84:16 acetonitrile:di water in 880 µl di water, Spike concentrations: B1 = 16 ppb; B2 = 4 ppb; G1 = 16 ppb; B2 = 4 ppb
injection 20 µl
detector FLD excitation = 360 nm, emission = 440 nm


Analysis Note Aflatoxins are a complex of closely related toxins produced as secondary metablolites of fungi. They are found in grains and nuts such as corn, wheat, or peanuts. They are produced by aspergillus flavus and a. Parasiticus. Members of the group include aflatoxin b1, aflatoxin b2, aflatoxin g1, aflatoxin g2, aflatoxin m1, and aflatoxin m2.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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