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HPLC Analysis of Aflatoxins in Peanut Paste on Ascentis® Express C18 following SPE using Supel™ Tox AflaZea

HPLC Analysis of Aflatoxins in Peanut Paste on Ascentis® Express C18 following SPE using Supel™ Tox AflaZea

Conditions

sample preparation Solid Phase Extraction
sample/matrix raw peanut paste
extraction process suspend 25 g raw peanut paste in 100 mL acetonitrile:water (84:16) extraction solution
SPE tube/cartridge Supel Tox AflaZea, 6 mL (55314-U)
sample addition directly load 2 mL extract onto SPE tube
elution pull sample through the SPE tube by vacuum at a rate of 8 ml/min
eluate post-treatment combine 200 µl eluate and 300 µl of water:trifluoroacetic acid:acetic acid (70:20:10 ) derivatization reagent, (heat at 65C for 25 min, add 500 µl of water to derivatized sample and vortex 30 sec)
column Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (53825-U)
column temp. 35 C
mobile phase [A} acetonitrile; {B} water; (20:80, A:B)
flow rate 0.2 ml/min
sample B1, G1 (8 ppb), B2, G2 (2 ppb)
injection 20 µl
detector fluorescence, ex 365 nm, em 450 nm

Description

Analysis Note Aflatoxins are carcinogenic metabolites produced by fungi that infect major agricultural commodites. The presence and concentrations of aflatoxins is widely regulated. Here, a cleanup method using interference removal SPE followed by analysis by HPLC with fluorescence detection is used to analyze derivatized samples extracted from raw peanut paste. The HPLC method was optimized for fluorescence detection and quantification of aflatoxins at < 10 ppb concentrations.
Categories Analytical Chromatography, SPE Applications, Mycotoxins
Featured Industry Agriculture
Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
Other Notes Sample pretreatment: suspend 25 g raw peanut paste in 100 mL acetonitrile:water (84:16) extraction solution
suitability application for SPE, application for HPLC

Materials

     
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