HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea

HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 25 g wheat meal spiked with pesticide standards
derivatization via KOBRA electrochemical cell, 50 muA
SPE tube/cartridge Supel(TM)Tox AflaZea, 6 mL (55314-U)
sample addition 2 mL
elution collect eluate in 5 mL silanized culture tubes
eluate post-treatment combine 200 µl of sample eluate and 880 µl of DI water in a 2 mL autosampler vial
column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 35 C
mobile phase [A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 µL/L nitric acid
flow rate 0.400 ml/min
pressure 4568 psi (315 bar)
injection 20 µl
detector FLD 360 ex; 440 em

Description

Analysis Note Sample/matrix:
Weigh 25 grams of wheat meal into a 500 mL Erlenmeyer flask. Add 100 mL of 84:16 acetonitrile:water, seal, and place on an orbital shaker at med-high speed for 90 minutes. Filter the sample through a #2 qualitative filter. Spike sample at 2 mg/mL using analytical standards.
Categories Analytical Chromatography, SPE Applications, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links