HPLC Analysis of Aldehydes and Ketones as DNPH Derivatives on Ascentis® Express C18

HPLC Analysis of Aldehydes and Ketones as DNPH Derivatives on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 µm particles (53827-U)
column temp. 30 °C
mobile phase [A] water; [B] acetonitrile; (40:60, A:B)
flow rate 2 mL/min
pressure 4685 psi (323 bar)
sample Sample in water
injection 5 µL
detector UV, 360 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the separation of aldehyde/ketone-DNPH derivatives.
Categories Analytical Chromatography, Air Monitoring Applications, Aldehydes, Aldehydes & Ketones
Featured Industry Industrial Hygiene
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for air monitoring, application for HPLC

Materials

     
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