HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)
flow rate 1.0 mL/min
injection 5 µL
detector UV, 280 nm

Description

Analysis Note This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented
Categories Analytical Chromatography, Antineoplastics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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