HPLC Analysis of Aminopterin and Related Compounds on Ascentis® RP-Amide

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565352-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)
flow rate 1.0 mL/min
injection 10 µL
detector UV, 280 nm

Description

Analysis Note This application demonstrates the suitability of the Ascentis RP-Amide for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis RP-Amide are presented.
Categories Analytical Chromatography, Antineoplastics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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