HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C18

HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C18

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 µm particles (568222-U)
column temp. ambient
mobile phase [A] (10 mM NH4OAc, pH 7):(50% CH3CN in 20 mM NH4OAc, pH 7), 65:35 ; [B] (10 mM NH4OAc, pH 7):(50% CH3CN in 20 mM NH4OAc, pH 7), 25:75
gradient 0-100% B in 12.5 min
flow rate 1 mL/min
injection 6 µL(10 mug each) in water
detector UV, 254 nm

Description

Categories Analytical Chromatography, Hormones
Featured Industry Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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