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HPLC Analysis of Angiotensins on Ascentis® RP-Amide

HPLC Analysis of Angiotensins on Ascentis® RP-Amide


column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase [A] 5 mM ammonium phosphate (pH 7.0 with ammonium hydroxide); [B] acetonitrile; (77.5:22.5, A:B)
flow rate 1.0 ml/min
sample as indicated in mobile phase
injection 10 µl
detector UV, 215 nm


Categories Analytical Chromatography, Pharmaceutical Analysis, Angiotensins, Hormones
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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