HPLC Analysis of Antibiotics on Ascentis® C18

HPLC Analysis of Antibiotics on Ascentis® C18

Conditions

column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 C
mobile phase [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetonitrile (93:7, A:B)
flow rate 1 ml/min
sample 50 µg/mL each in mobile phase
injection 10 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Antibiotics, Antimicrobials, Antibiotics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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