HPLC Analysis of Apotransferrin Tryptic Digest on BIOshell™ A160 Peptide C18, Comparison of Different Flow Rates and Pressures

HPLC Analysis of Apotransferrin Tryptic Digest on BIOshell™ A160 Peptide C18, Comparison of Different Flow Rates and Pressures

Conditions

column BIOSHELL A160 Peptide C18, 15 cm x 4.6 mm I.D., 5 µm particles (67015-U)
column temp. 60 °C
mobile phase [A] water/0.1% TFA [B]: acetonitrile/0.1% TFA
gradient 5-50%B in 30 minutes; 5-50%B in 15 minutes;
flow rate 2 mL/min or 4/mL minute
pressure 126 bar, 250 bar
sample 1 mg/mL in water/0.1% TFA
injection 15 µL
detector UV, 215 nm

Description

Analysis Note BIOshell columns packed with five micron Fused-Core particles feature modest back pressures, which allows the use of very high flow rates as is demonstrated in the two chromatograms, one of which was obtained at 2mL/min, the other at 4mL/min. Note that at 4mL/min the gradient time was reduced in half to keep the gradient volume constant. At a flow rate of 4mL/min the back pressure for this 15cm x 4.6mm column is 250 bar (approx. 3500 psi), which illustrates that these high efficiency columns can be operated on standard HPLC systems.
Categories Analytical Chromatography, Pharmaceuticals, Peptides
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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