HPLC Analysis of Artificial Food Colorants on Chromolith® HighResolution RP-18 endcapped, UV 428 nm

HPLC Analysis of Artificial Food Colorants on Chromolith® HighResolution RP-18 endcapped, UV 428 nm

Conditions

column Chromolith High Resolution RP-18, 10 cm x 4.6 mm, 2 µm particles (1.52022.0001)
column temp. 30 °C
mobile phase [A] 20mM ammonium acetate pH 6.8 in water; [B] methanol
gradient 5 to 50% B in 6 min; to 100% B in 4 min; held at 100% B for 1 min; to 5% B in 0.01 min
flow rate 1 mL/min
pressure 1526 psi (106 bar)
sample Tartrazine 0.05 mg/mL, Amaranth 0.05 mg/mL, Carmine Red 0.05 mg/mL, Sunset Yellow 0.05 mg/mL, Fancy Red 0.05 mg/mL in methanol:water (50:50)
inj. temp. 20 µL
detector UV, 428 nm

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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