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HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide

HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide


column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase 25 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid)
flow rate 1 ml/min
sample as indicated each in mobile phase
injection 10 µl
detector UV, 230 nm


Categories Analytical Chromatography, Organic Acids
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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