HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide

HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 µm particles (53929-U)
column temp. 30 °C
mobile phase [A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA; (65:35, A:B)
flow rate 1 mL/min
pressure 2200 psi (152 bar)
sample 50 µg/mL in 75:25, water:acetonitrile
injection 5 µL
detector UV, 250 nm

Description

Analysis Note Salicylic acid is the primary metabolite of aspirin, and is likely responsible for its antiinflammatory properties via suppression of cyclooxygenase genes; it also is thought to suppress colonic carcinogenesis. Ascentis Express RP-Amide provides good retention and resolution at low pH by a combination of hydrophobic and H-bonding intereactions.
Categories Analytical Chromatography, Anti-inflammatories, Analgesics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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