HPLC Analysis of B Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase

HPLC Analysis of B Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase

Conditions

column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50773-U)
column temp. 30 C
mobile phase [A] 0.02 M Ammonium Formate, pH = 3.0; [B] acetonitrile
gradient 0-50% B in 1 min. Hold at 50% B until 1.5 min.
flow rate 1.5 ml/min
pressure 1639 psi (113 bar)
sample dissolved in 50/50 water/acetonitrile
injection 1 µl
detector UV, 276 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Food and Beverages
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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