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HPLC Analysis of B-Vitamins in Vitamin Water using Ascentis® Express RP-Amide and UV Detection

HPLC Analysis of B-Vitamins in Vitamin Water using Ascentis® Express RP-Amide and UV Detection

Conditions

column Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 µm particles (53918-U)
column temp. 30 C
mobile phase [A] 20 mM potassium phosphate, pH 3; [B] methanol
gradient held at 0.5% B for 1.5 minutes, to 25% B in 1.0 minutes, held at 25% B for 1.7 minute, held at 0.5% B for 1.2 minutes
flow rate 0.75 ml/min
pressure 5584 psi (385 bar)
sample vitamin water was filtered through 0.2um nylon syringe filter
injection 0.5 µl
detector UV, 210 nm

Description

Analysis Note The vitamin water drink was filtered using 0.2 um Nylon filter and injected into UHPLC for B-vitamin analysis
Categories Analytical Chromatography, Pharmaceutical Analysis, Food & Beverage Analysis, Vitamins and Natural products, Beverage Testing, Anticonvulsant/antiepiletic/antiseizure, Sports and Energy Drinks
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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