HPLC Analysis of Basic Peptides (Angiotensin Analogs) on BIOshell™ A160 Peptide C18

HPLC Analysis of Basic Peptides (Angiotensin Analogs) on BIOshell™ A160 Peptide C18

Conditions

column BIOshell A160 Peptide C18, 10 cm x 3 mm I.D., 2.7 µm particles (66908-U)
column temp. 35 °C
mobile phase [A] 0.1% (v/v) formic acid, pH 4.0 (titrated with ammonium hydroxide); [B] 50:50, (0.175% formic acid in water, pH 4.0) : acetonitrile
gradient 20 to 50% B in 15 min
flow rate 0.6 mL/min
pressure 3002 psi (207 bar)
sample 0.5 g/L ea. peptide
injection 2.0 µL
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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