HPLC Analysis of Basic Peptides on Discovery® BIO Wide Pore C18, No TFA

HPLC Analysis of Basic Peptides on Discovery® BIO Wide Pore C18, No TFA

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 2.1 mm I.D., 5 µm particles (568202-U)
column temp. 35 °C
mobile phase [A] 25 mM formic acid in water; [B] 50:50 (25 mM formic acid in water):(20 mM formic acid in acetonitrile)
flow rate 0.20 mL/min
injection 0.5 µL (~0.25 mug each peptide)
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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