HPLC Analysis of Benzoate and Sorbate on Ascentis® Express RP-Amide

HPLC Analysis of Benzoate and Sorbate on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 µm particles (53916-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)
flow rate 0.4 mL/min
sample 50 mg/L each
injection 1 µL
detector UV, 215 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of sorbate and benzoate.
Categories Analytical Chromatography, Preservatives
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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