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HPLC Analysis of Benzodiazepines on Ascentis® C8

HPLC Analysis of Benzodiazepines on Ascentis® C8


column Ascentis C8, 15 cm x 2.1 mm I.D., 3 µm particles (581402-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium acetate (pH 6.98 unadjusted); [B] acetonitrile; (55:45, A:B)
flow rate 0.2 ml/min
sample 1 µg/mL each in 10 mM ammonium acetate (pH 6.98 unadjusted)
injection 5 µl
detector MS, ESI (+), Selected Ion Recording Mode


Categories Analytical Chromatography, Clinical, Pharmaceutical Analysis, psychotropics, Anticonvulsant/antiepiletic/antiseizure, antiepileptic/antiseizure, Psychotropics
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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