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HPLC Analysis of Beta Blockers on Ascentis® Si (Silica)

HPLC Analysis of Beta Blockers on Ascentis® Si (Silica)


column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 35 C
mobile phase [A] 0.1% ammonium acetate in water (pH unadjusted); [B] 0.1% ammonium acetate in acetonitrile; (15:85, A:B)
flow rate 1 ml/min
sample as indicated in 0.1% ammonium acetate in 10:90:water:acetonitrile
injection 10 µl
detector UV, 225 nm


Categories Analytical Chromatography, Clinical, antihypertensives, beta-blockers, Antihypertensives
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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