HPLC Analysis of Beta Blockers on Ascentis® Express C18 using a 60 Second Gradient

HPLC Analysis of Beta Blockers on Ascentis® Express C18 using a 60 Second Gradient

Conditions

column Ascentis Express C18, 2 cm x 2.1 mm I.D., 5 µm particles (50507-U)
column temp. 40 °C
mobile phase [A] water/0.1% TFA; [B] acetonitrile/0.1% TFA
gradient 5-50% B in 60 seconds
flow rate 2 mL/min
sample 0.17-0.33 mg/mL in 71% water/29% acetonitrile
injection 0.5 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Beta-blockers
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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