HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express C18

HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U)
column temp. 40 C
mobile phase [A] 20 mM ammonium acetate, pH 4.7 with acetic acid: [B] acetonitrile; (90:10, A:B)
flow rate 0.6 ml/min
sample 100 - 500 µg/mL in 95:5, buffer: acetonitrile
injection 1 µl
detector UV, 214 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.
Categories Analytical Chromatography, Food & Beverage Analysis, Sweeteners, Stiµlants, Beverage Testing, Carboxylic acids, Soft Drinks
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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