HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express RP-Amide

HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 µm particles (53918-U)
column temp. 40 C
mobile phase [A] 20 mM ammonium acetate, pH 4.7 with acetic acid; [B] acetonitrile; (92:8, A:B)
flow rate 0.6 ml/min
sample 500 - 1000 µg/mL in 95:5, buffer: acetonitrile
injection 1 µl
detector UV, 214 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola.
Categories Analytical Chromatography, Food & Beverage Analysis, Carboxylic acids, Beverage Testing, Stiµlants, Sweeteners, Soft Drinks
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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