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HPLC Analysis of Biogenic Amines on Ascentis® Si, HILIC Mode (85 % Acetonitrile)

HPLC Analysis of Biogenic Amines on Ascentis® Si, HILIC Mode (85 % Acetonitrile)

Conditions

column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 35 C
mobile phase [A] 0.1% ammonium acetate in water (pH unadjusted); [B] 0.1% ammonium acetate in acetonitrile; (15:85, A:B)
flow rate 1 ml/min
sample 50 µg/mL in 0.1% ammonium acetate in 10:90:water:acetonitrile
injection 10 µl
detector UV, 280 nm

Description

Categories Analytical Chromatography, Clinical, amines, Amines
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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