HPLC Analysis of Bisphenol A in Water on Ascentis® Express C18 after SPE using Supelclean™ ENVI-18

HPLC Analysis of Bisphenol A in Water on Ascentis® Express C18 after SPE using Supelclean™ ENVI-18

Conditions

SPE tube/cartridge Supelclean ENVI-18, 500 mg/6 mL glass SPE tube (54331-U)
condition 1 mL 1% formic acid in acetonitrile, then 1 mL water
elution 2 mL 1% formic acid in acetonitrile
eluate post-treatment 1 mL aliquot of eluent, and evaporate/reconstitute to 0.5 mL in acetonitrile
column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 35 °C
mobile phase [A] water; [B] acetonitrile; (60:40, A:B)
flow rate 0.4 mL/min
pressure 3270 psi (256 bar)
sample 5 mL spiked water blank, concentration of 0.2 µg/mL
injection 1 µL
detector UV, 230 nm

Description

Analysis Note Bisphenol A is a contaminant that can leach into aqueous solutions from polycarbonate plastics or metal cans; it may impact human development and reproduction. This application demonstrates the extraction and analysis of bisphenol A from water.
Categories Analytical Chromatography, Food & Beverage Analysis, Environmental Analysis, Beverage Testing, Phthalate Esters, Organics, Drinking Water and Bottled Water, Chromatograms, HPLC Chromatograms
Featured Industry Environmental
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supelclean is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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