HPLC Analysis of β-Blockers and β-Agonists in Urine and Wastewater on a C18 Column after SPE using SupelMIP® SPE-Beta-Receptor

HPLC Analysis of β-Blockers and β-Agonists in Urine and Wastewater on a C18 Column after SPE using SupelMIP® SPE-Beta-Receptor

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix horse urine or waste water spiked with 10 beta-agonists and beta-blockers at 1 ng/mL
SPE tube/cartridge SupelMIP SPE - Beta-Receptor, 25 mg/10 mL (LRC) (53223-U)
condition 1 mL acetonitrile; 1 mL DI water
sample addition 1 mL
washing 3 x 1 mL DI water; 1 mL acetonitrile; 1 mL 60% acetonitrile/40% DI water; apply 2 min of full vacuum to dry the tube after each step
elution 2 x 1 mL 1% formic acid in acetonitrile
eluate post-treatment evaporate under nitrogen and reconstitute with 150 µL 5% acetonitrile in 10 mM ammonium acetate, pH 4.6 prior to LC-MS-MS analysis
column C18, 5 cm x 3 mm I.D., 3 µm particles
column temp. ambient
mobile phase [A] 10 mM ammonium acetate, pH 4.6 (adjusted with acetic acid); and [B] acetonitrile
flow rate 0.5 mL/min
injection 20 µL
detector MS/MS, ESI(+)

Description

Application Sample Pre-treatment:
Horse urine was centrifuged at 3000 g for 10 min, diluted 1:1 (v/v) with DI water, adjusted to pH 7. Wastewater was filtered with 1 μm filter paper and adjusted to pH 6-7. Samples were spiked with 10 beta-agonists and beta-blockers at the level of 1 ng/mL.
Categories Analytical Chromatography, Environmental Analysis, Clinical, SPE Applications, beta-agonists, bronchodilators, Pharmaceutical & Clinical applications, Antihypertensives, antihypertensives, Organics, Chromatograms, HPLC Chromatograms
Featured Industry Clinical
Environmental
Legal Information SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for SPE

Materials

     
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