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HPLC Analysis of Bufalin Toxin on Discovery® BIO Wide Pore C18

HPLC Analysis of Bufalin Toxin on Discovery® BIO Wide Pore C18

Conditions

column Discovery BIO Wide Pore C18, 25 cm x 4.6 mm I.D., 5 µm particles (568223-U)
column temp. ambient
mobile phase [A] 0.1% TFA in water; [B] 0.1% TFA in acetonitrile:water (3:1, A:B)
flow rate 1.3 ml/min
injection 10 muLbufalin, 0.5 mg/mL in acetonitrile
detector UV, 298 nm

Description

Categories Analytical Chromatography, Forensics and Toxicology, steroids, Steroids
Featured Industry Forensics and Toxicology
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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