HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

Conditions

column Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 µm particles (53967-U)
column temp. 25 °C
mobile phase [A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B)
flow rate 0.6 mL/min
sample 1000 µg/mL in 10:90, water: acetonitrile
injection 2 µL
detector ELSD, 55 °C, 3.5 bar nitrogen

Description

Analysis Note This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.
Categories Analytical Chromatography, StiµLants, Organic Acids, Drugs of Abuse
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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