HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery® RP-Amide C16

HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery® RP-Amide C16

Conditions

column Discovery RP-AmideC16, 25 cm x 4.6 mm I.D., 5 µm particles (505013)
column temp. 35 °C
mobile phase [A] 0.1% TFA, 1% acetonitrile in water; [B] 0.1% TFA, 40% acetonitrile
gradient 0-100% B in 130 min
flow rate 1 mL/min
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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