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HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si

HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si

Conditions

column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 30 C
mobile phase [A] hexane; [B] ethyl acetate; [C] isopropanol; (85:10:5, A:B:C)
flow rate 1.0 ml/min
sample 50-100 µg/mL hexane
injection 10 µl
detector UV, 450 nm

Description

Analysis Note Ascentis silica is used in the analysis of naturally occurring carotenoid pigments in the form of alcohols, xanthophyll and astaxanthin.
Categories Analytical Chromatography, Pigments, Hydrocarbons
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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