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HPLC Analysis of Catechins on Ascentis® RP-Amide

HPLC Analysis of Catechins on Ascentis® RP-Amide


column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium formate (pH 3.0 with concentrated formic acid); [B] acetonitrile
flow rate 1 ml/min
sample 10 µg/mL each in water:acetonitrile 1:1
injection 5 µl
detector MS, ESI (-) in selected ion recording (SIR) mode


Categories Analytical Chromatography, Antioxidants
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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