HPLC Analysis of Cetirizine and Hydroxyzine on Ascentis® Express F5

HPLC Analysis of Cetirizine and Hydroxyzine on Ascentis® Express F5

Conditions

column Ascentis Express F5, 10 cm x 3.0 mm I.D., 2.7 µm particles (53578-U)
column temp. 25 °C
mobile phase 5 mM ammonium formate pH 3.0 with formic acid in 90:10, acetonitrile:water
flow rate 0.6 mL/min
pressure 1300 psi (90 bar)
sample 50 µg/mL in 90:10, acetonitrile:water
injection 2 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Antihistamines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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